thom med

Sample positioning in the petri dish

Aga Cup

An agarose cup placed over tissue or cell samples. Its flat base keeps the sample localized on the dish floor while medium diffuses through the agarose.

Construction principle

Geometry controls position and diffusion.

Inner well for culture medium. Thin agarose base over the sample. Three wings stabilize placement in the culture dish.

  1. 01

    Medium reservoir

    The inner well holds culture medium; soluble factors diffuse through the agarose body to the sample.

  2. 02

    Thin agarose base

    The sample-facing base rests over the sample and defines a short diffusion path.

  3. 03

    Positioning wings

    Three wings stabilize placement in the culture dish and keep the cup repeatably over the sample.

Problem and workflow

Localize the sample. Feed by diffusion.

The Aga Cup is placed over a tissue or cell sample in the petri dish. The sample stays localized; medium reaches it by diffusion through agarose.

Without cup
The sample drifts in medium.
With cup
The flat agarose base holds it on the dish floor.
Medium
Pipette into the inner well; diffusion to the sample.
After localization
Lift the cup away; the sample remains defined in position.
Aga Cup made from translucent agarose, held with tweezers
Real Aga Cup: translucent agarose, handled with standard tweezers.
Tissue sample drifting in medium inside a petri dish
Without localization, the sample drifts in medium.
01 / Position sample

Sample on the dish floor

The tissue or cell sample is placed on the defined culture area; the empty dish is the starting state.

  1. A

    Sample on the dish floor

  2. B

    Defined culture area

01 / Position sample

Sample on the dish floor

The tissue or cell sample is placed on the defined culture area; the empty dish is the starting state.

  1. A

    Sample on the dish floor

  2. B

    Defined culture area

02 / Place cup over sample

Sample under the flat base

The Aga Cup is placed over the sample; the flat agarose base localizes it on the dish floor.

  1. A

    Flat agarose base over the sample

  2. B

    Sample under the cup

  3. C

    Positioning wings near the dish wall

03 / Fill medium into the inner well

Reservoir and diffusion path

Medium is pipetted into the inner well and diffuses through the agarose body to the sample.

  1. A

    Medium reservoir in the inner well

  2. B

    Diffusion path through agarose

  3. C

    Sample remains on the dish floor

04 / Lift cup after localization

Sample remains localized

After attachment or localization, the cup can be lifted away; the sample remains in a defined position.

  1. A

    Localized sample

  2. B

    Dish ready for further culture

Technical context

Material and culture mode.

Material, culture mode and available documentation for initial technical assessment. Protocols and application-specific dimensions are coordinated during the sample request.

Request data sheet
Base material
Agarose, animal-component-free
Gel concentration
1.5–3.0 %, adjustable to application
Biocompatibility
For in-vitro research applications; biocompatibility context available on request
Sterilization
Sterilization and handling protocol available on request for the specific application
Culture mode
Tissue samples, cell clusters, spheroids and organoids under a diffusion-fed carrier
Geometry
Cup with medium-filled inner well and flat sample-facing base

Sample request

Check your workflow fit.

Describe sample type, dish format and culture goal. Thom-Med will respond with sample availability and technical context.

Request Sample